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KMID : 1101420200520030245
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Korean Journal of Clinical Laboratory Science
2020 Volume.52 No. 3 p.245 ~ p.252
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Cathepsin B Is Implicated in Triglyceride (TG)-Induced Cell Death of Macrophage
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Jung Byung-Chul
Lim Jae-Won
Kim Sung-Hoon
Kim Yoon-Suk
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Abstract
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Macrophage cell death contributes to the formation of plaque, leading to the development of atherosclerosis. The accumulation of triglyceride (TG) is also associated with the pathogenesis of atherosclerosis. A previous study reported that TG induces the cell death of macrophages. This study examined whether the cytoplasmic release of cathepsin B from lysosome is associated with the TG-induced cell death of macrophage. The release of cathepsin B was increased in the TG-treated THP-1 macrophages, but the TG treatment did not affect cathepsin B expression. Furthermore, the inhibition of cathepsin B by its inhibitor, CA-074 Me, partially inhibited the TG-induced cell death of macrophage. TG-triggered macrophage cell death is mediated by the activation of caspase-1, -2, and apoptotic caspases. Therefore, this study investigated whether cathepsin B is implicated in the activation of these caspases. The inhibition of cathepsin B blocked the activation of caspase-7, -8, and -1 but did not affect the activity of caspase-3, -9, and -2. Overall, these results suggest that TG-induced cytoplasmic cathepsin B causes THP-1 macrophage cell death by activating caspase-1, leading to subsequent activation of the extrinsic apoptotic pathway.
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KEYWORD
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Caspase-1, Cathepsin B, Cell death, THP-1 macrophage, Triglyceride
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